pcdna3 sec61b v5 apex Search Results


pcdna5 sec61b v5 turboid  (Addgene inc)
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Addgene inc pcdna5 sec61b v5 turboid
Pcdna5 Sec61b V5 Turboid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 sec61b v5 apex2 vector  (Addgene inc)
93
Addgene inc pcdna3 sec61b v5 apex2 vector
Pcdna3 Sec61b V5 Apex2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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promotor vector details tom20v5turboid kpni tom20bamhi v5 nheibira  (Addgene inc)
93
Addgene inc promotor vector details tom20v5turboid kpni tom20bamhi v5 nheibira
Promotor Vector Details Tom20v5turboid Kpni Tom20bamhi V5 Nheibira, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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sec61b apex2  (Addgene inc)
92
Addgene inc sec61b apex2
( A ) Proximity labeling of IRE1α. HEK293 cells expressed <t>S1R-APEX2</t> and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).
Sec61b Apex2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+sec61b+v5+apex/pCDNA3_V5-APEX2-Sec61B+(Plasmid+%2383412)/pmc08112866-53-4-17
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sec61b apex2 - by Bioz Stars, 2026-10
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c1 1 29 turboid v5 pcdna3 vectors  (Addgene inc)
94
Addgene inc c1 1 29 turboid v5 pcdna3 vectors
( A ) Proximity labeling of IRE1α. HEK293 cells expressed <t>S1R-APEX2</t> and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).
C1 1 29 Turboid V5 Pcdna3 Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+sec61b+v5+apex/pAAV-TBG-Cyto-TurboID+(Plasmid+%23149414)/bio_rxiv__2024__06__19__599752-336-5-10
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addgene pasmid  (Addgene inc)
95
Addgene inc addgene pasmid
( A ) Proximity labeling of IRE1α. HEK293 cells expressed <t>S1R-APEX2</t> and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).
Addgene Pasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+sec61b+v5+apex/V5-TurboID-NES_pCDNA3+(Plasmid+%23107169)/pm35609616-256-263-263
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mcherrymyr5  (Addgene inc)
93
Addgene inc mcherrymyr5
( A ) Proximity labeling of IRE1α. HEK293 cells expressed <t>S1R-APEX2</t> and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).
Mcherrymyr5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+sec61b+v5+apex/pcDNA3%2E1_Lifeact-mCherry+(Plasmid+%2367302)/pm38815583-284-40-55
Average 93 stars, based on 1 article reviews
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Image Search Results


( A ) Proximity labeling of IRE1α. HEK293 cells expressed S1R-APEX2 and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).

Journal: eLife

Article Title: The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains

doi: 10.7554/eLife.65192

Figure Lengend Snippet: ( A ) Proximity labeling of IRE1α. HEK293 cells expressed S1R-APEX2 and Sec61β-APEX2 or APEX2-KDEL as controls. Cells were incubated with biotin-phenol, and proteins were labeled by a 1 min addition of H 2 O 2 . Biotinylated proteins were pulled down and analyzed by western blot. ( B ) Western blot analysis of lysates prepared from control HEK293 cells, S1R KO HEK293 cells, and both cell lines transfected with S1R-6His construct as indicated. ( C ) Time-dependence of the thapsigargin (Tg)-induced IRE1α response in WT and S1R KO HEK293 cells. Western blot is representative of n = 3 independent experiments. ( D ) Quantification results of the western blot shown above. p-IRE1α levels were normalized to the total level of IRE1α and plotted as mean (solid line) ± SEM (shaded) from n = 3 independent experiments for WT (black) and S1R KO cells (red). ( E ) Quantification results for area under the curve of the Tg-induced p-IRE1α response. p-value=0.015 based on t-test. ( F ) Quantification results of the western blot results for XBP1s. Intensities of protein bands were normalized to tubulin and plotted as mean ± SEM from n = 3 independent experiments for WT (black) and S1R KO cells (red).

Article Snippet: Recombinant DNA reagent , Sec61b-APEX2 , This study , , Generated by cloning Sec61b-APEX fusion gene (RRID: Addgene_83411 ) into lentivector.

Techniques: Labeling, Incubation, Western Blot, Transfection, Construct

Journal: eLife

Article Title: The role of sigma 1 receptor in organization of endoplasmic reticulum signaling microdomains

doi: 10.7554/eLife.65192

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Sec61b-APEX2 , This study , , Generated by cloning Sec61b-APEX fusion gene (RRID: Addgene_83411 ) into lentivector.

Techniques: Generated, Selection, Isolation, Recombinant, Plasmid Preparation, Mutagenesis, Construct, Clone Assay, Sequencing, Software